S.H.I.E.L.D. is a Marvel Comics fictional advanced counterterrorism and intelligence agency charged with investigating and neutralizing paranormal and superhuman threats for global security. It was created by Stan Lee and Jack Kirby in #135 (August 1965) and appeared throughout the publisher's entire Strange Tales comic book line. The agency and its traditional executive director, Nick Fury, have also starred in their own series and miniseries sporadically since that time, most notably during a late-1960s run by writer-artist Jim Steranko.
continuity versions of S.H.I.E.L.D. are listed here: the traditional version in mainstream Marvel Universe continuity, followed by the alternate reality version published under the Ultimate Marvel imprint, as well as the Marvel Cinematic Universe version. Amalgam Comics, a joint venture between Marvel and DC Comics, have their own version of S.H.I.E.L.D. that combines characters from both publishers.
Marvel Universe S.H.I.E.L.D
Executive Directors and Secretaries
Notable Agents (Pre-Civil War)
Agent 22 
Agent 74 – Sent to battle Vamp to demonstrate her abilities to  Captain America.
Agent M – Expert in the  Microverse.
John Allen Adams – Head of  ESP squad.
Agent Boyer undercover unit, dating Joe
Dr. Ames – Medical surgeon stationed at Central. 
Earl Angstrum – Senior agent. His son was killed by the mutation of former  Deathlok John Kelly (Biohazard).
Artie – Member of S.H.I.E.L.D.'s Superhuman Cleanup Department of Sanitation (SCUDS). 
Ashton – He is in charge of securing the alien spaceship of Golden Blade and Sapper. 
Ken Avery – Thirty-year veteran. A senior officer aboard the  Helicarrier.
Balaban – Serving on the Helicarrier with  G. W. Bridge.
Baker – Stationed aboard the Helicarrier. 
Ted Bailey – Stationed at front company D-Fleks Industries. Briefly carried consciousness of  Jack Truman.
Beefcake – LMD assigned to the Hulkbusters unit.
Bradley Beemer – Part of the Howling Commandos monster force. He is also the Area 13 technical chief. 
Bellini – Ranking officer at the  Venice, Italy, station.
Berger – Formerly stationed in a training camp in  Saudi Arabia. She accompanied Nick Fury to investigate a base in the Middle East.
Bill – Partner of Joe. He picked up Agent L's microfilm from unnamed agents, but blew up his own car to stop HYDRA agents. It is yet to be confirmed whether he survived or not. 
Sally Blevins (Skids) – A  mutant who infiltrated two factions of the Morlocks, later involved in intrigue among Hawkeye, the Winter Soldier and Black Widow. 
Boothroyd – Administrator in charge of personal effects department. Probable transfer from MI-6. Could also be a reference to Major Boothroyd, the equipment officer code-named "Q" in the  James Bond movie series.
Abigail Brand – Head of  S.W.O.R.D. (Sentient World Observation and Response Department), a subdivision of S.H.I.E.L.D. She is a hybrid mutant and alien.
Joseph Bricklemoore – Agent of the Mutant Task Force that infiltrated the Jean Grey School Student Body by using MGH (Mutant Growth Hormone) to become the mutate Tri-Joey.
Josephine Bricklemoore – Agent of the Mutant Task Force that infiltrated the Jean Grey School Student Body by using MGH (Mutant Growth Hormone) to become the mutate Squidface.
Bubba – Member of S.H.I.E.L.D.'s Alpha Team. 
Barth Bukowski – Regional Director in Los Angeles. 
Jenna Carlisle – Forensics agent and adrenaline junkie.
Mitch Carson – Security agent under  Dum Dum Dugan.
Carstairs – Member of the Alpha Team Armored Squad.
– Chief of S.H.I.E.L.D.'s Artificial Intelligence division. Monica Chang
Cheesecake – LMD assigned to the Hulkbusters unit.
Isadore "Izzy" Cohen – Former  private first class in Fury's World War II squad. Agent of S.H.I.E.L.D.
Colburn – Agent who noted traffic on the Project Contingency files at HQ. 
Colletti – Member of the Alpha Team Armored Squad.
William Collins – Division commander who led the battle to raze the slave-camp island of the  Red Skull and the Hate-Monger.
- Also known as the Agent, he is the main Agent of the Marvel Cinematic Universe. Phil Coulson
Crimson – Head of Magic-Ops division and assigned to the Hulkbusters unit. 
Cross – Friend of Contessa Valentina. 
Valentina Allegra de Fontaine  – Former Special Director at Public Relations. Senior liaison officer to  MI5. Level 9 agent. Revealed to have been replaced by a Skrull.
Jessica Drew – The original  Spider-Woman. Level 7 agent.
Stanley Dreyfuss –  Elektra's contact on Operation: Lock Kiss. Later learns he is a Life Model Decoy.
Joanie "Nails" Eaton – Member of the Elite Agents. 
John Facchino –  Human resources department.
Rigby Fallon – Boy genius in the  Artificial Intelligence division. Wrote most of the programs for the latter-day Helicarrier.
Farrell – Member of the Alpha Team Armored Squad.
E.B. Farrell – Weapons expert for the Elite Agents. Calls himself "the Kid". 
Fisher – Dawn Helicarrier technician. 
Nick Fury, Jr. – Son of Nick Fury. Also known as  Marcus Johnson.
Gerrard – Field agent for the Foreign Affairs office. 
Giulietta – Technician at the Venice, Italy, station. 
Gomez – Member of the Alpha Team Armored Squad.
Herrick Goldman – Among group of renegade agents attempting to take over the Helicarrier for the impromptu  war crimes trial of a KGB agent.
Hazeltine – Intelligence agent at Central. 
Hugh Howard – Pilot and mechanic aboard Behemoth IV Helicarrier, charged with capturing  Godzilla. The name is a reference to aviator, engineer, and multi-billionaire Howard Hughes.
Jerry Hunt – Agent assigned to  Scotland Yard. Former lover of Jessica Drew.
Horatio Huxley – Ranking executive of Level 13. He was previously involved in  Alpha Flight.
Jackson – Pilot in Unit 6 of the Air Cavalry. 
Karl Janáček – Rank L-3. 
Valerie Jessup Toomes – Daughter of supervillain the  Vulture.
Johnson – Attempted to free agents from the City of the Space Gods. 
Daisy Johnson – Seismic-powered "super-agent". She is the only known agent with "Level 10" security clearance aside from Fury and the Black Widow (Natasha Romanova). 
Jones (Jonesy) – Youthful field agent involved in the operations against the  Pantheon and the Punisher. 
Gabriel "Gabe" Jones – Former  private first class in Fury's World War II squad.
Kallebach – Field agent during investigation of assassinations at J-2 conference. 
Kelso – Pilot in Unit 6 of the Air Cavalry. 
Derek Khanata – Ex-Hatut Zeraze operative from  Wakanda. He was Carmilla Black's carrier and the senior investigator on the " Agents of Atlas" cases. After S.H.I.E.L.D. is dismantled, he later joins the group. 
Helen Kim – Agent investigating the Brothers Grace crime family. 
Veronica King 
Judith Klemmer (Agent 324) – Agent charged with tracking down Baron Ludwig von Schtupf, a.k.a. the Monster-Maker. 
Cameron Klein – Grade T-7 technician. Became field agent and helped capture supervillain Cache.  
Eric Koenig – German defector. He was a replacement member of the World War II  Howling Commandos.
Ali Kokmen – Interfered with Khanata in the Scorpion affair. 
Bruno Kreah – Low-level engineer. He worked on the  surveillance equipment used to infiltrate Datalink Systems.
Sayuri Kyota (M-80) –  Demolitions expert of the Elite Agents.
Sidney "Gaffer" Levine – Primary ordnance inventor and gadgeteer. 
– Charles Little Sky Mutant Director of A.R.M.O.R.
Alphonso "Mac" MacKenzie – Senior liaison officer to  CIA.
Dr. Myron MacLain – High-ranking scientist who is a seminal  adamantium researcher.
Dino Manelli – Former  private in Fury's World War II squad. He was an Italian-American star.
Kirby Martell – S.H.I.E.L.D. scientist. She operated a captured neo-Nazi time machine. 
Chastity McBryde – Squad leader. Fought against  Elektra and other rogues that S.H.I.E.L.D. suspected to be Elektra's accomplices.
Tony Masters – An agent of S.H.I.E.L.D. who would go on to become the Taskmaster. Because of his abilities, Masters has forgotten his S.H.I.E.L.D. past and unknowingly works as an undercover agent. 
Chris McCarthy – Low-level agent. He was the first person to wear  Hank Pym's 2006 Ant-Man suit.
Mercedes Merced – Undercover agent and Taskmaster's handler. 
Alisanda "Ali" Morales – Undercover in Cuba. She helped Captain America and  Falcon track down "Anti-Cap". Stayed with S.H.I.E.L.D. when it became H.A.M.M.E.R.
Murray – Agent working in reactor core of Central. 
Kate Neville - Aid to Nick Fury and trained marksman. Killed by  Baron Strucker. 
N'Gami – Technological advisor and a Wakandan government liaison officer. 
Noriko Nagayoshi – Tech Directorate under Agent Khantana during operation to outfit Camilla Black as the  Scorpion.
Niles Nordstrom – Rank L-8. 
Brady O'Brien – Nearly had affair with Mary Jane Parker when she was separated from Peter. He later attempted to force her to give up Spider-Man or face arrest. 
Eric O'Grady (Ant-Man) – Third known  Ant-Man.
Jake Oh – Field operative stationed at  Weapon Plus Headquarters.
Kara Lynn Palamas (Agent 33) – Researcher; recruited  Hercules for operation against Ares and Warhawks.
Jeffery Parks – Infiltrated the City of the Space Gods. He was reduced to basic matter. Rank L-6. 
Paulo – Technician at Venice station. 
Pandora Peters – Director of S.H.I.E.L.D.'s magic response unit, the Wizardry Alchemy and Necromancy Department W.A.N.D. 
Farrell Phillips – Commander for Dawn Helicarrier. Captain in Black Bird of the Gold Squadron. 
Mr. Postal – Mission coordinator for cyborg agents. 
Emily Preston – Agent whose human consciousness was transferred into a LMD after she was killed by zombie George Washington.
Clay Quartermain – Former liaison officer of the "  Hulkbusters", the Hulk-hunting operations of the U.S. Armed Forces. Supervisor for Nick Fury's Howling Commandos. Level 8 agent. Stated in transcripts of Nick Fury's "Secret Files" in . Secret War
Cliff Randall – Pilot. He later learned he was an  extraterrestrial.
Red (Agent 1–16) 
Steve Rogers (Captain America) – Has regularly undertaken missions for S.H.I.E.L.D. 
Jack Rollins – Infiltrated  Roxxon for the uncovered Delta program, the catalyst for Deltite Affair.
Natasha Romanova (Black Widow) – First known  Black Widow. Was a level 10 agent before S.H.I.E.L.D. was restructured. One of S.H.I.E.L.D.'s top agents.
Colonel Michael "Mickey" Rossi – Former lover of  Carol Danvers.
Gail Runciter – Trained alongside  Wendell Vaughn. She was temporarily replaced by a Deltite.
Sam – Stationed at Central's barbershop entrance. 
Ayna Sareva – Assisted Khanata in the Scorpion affair. 
Constance Seagrum – Pilot of Unit 6's Air Cavalry. 
Tia Senyaka – Investigated the death of Agent Harlan, who had died in a car accident. 
Gerald "Silcon" Simms – A member of the Elite Agents, with liquid-metal cybernetic arms. 
Simon – Communication officer on the Helicarrier. 
Captain Simon 
Jakuna Singh – Brother of Sarapha. Killed by Damiru.  
Jasper Sitwell – Interrogator. Former interim executive director and former liaison to  Tony Stark and Iron Man. Nick Fury's "Secret Files" in lists Sitwell as a Level 5 interrogator and a Level 5 agent in most transcripts. For two, he is listed as Level 8. Secret War
Carla Smith – Nick Fury's appointment secretary. 
Rosalind Solomon - Agent E-23. An environmental agent.
Dwight Rollin Stanford – Rank L-3. 
Michael Stevenson – Infiltrated the City of the Space Gods. He was reduced to basic matter. Rank L-6 
Angel Tarnaki – Air Cavalry. She brought in X-Force to reveal history of Dr. Constantin Racal and Niles Roman. 
Kimberly Taylor – One of the last agents trained by Nick Fury. Assigned by  G. W. Bridge to protect the Rev. William Connover.
Teresa – Fury's personal secretary. 
Colonel Nate Thurman – Chief administration officer for Helicarrier repairs. Among group of renegade agents attempting to take over Helicarrier for impromptu  war crimes trial of KGB agent.
Agent Todd - Direct report to Pandora Peters, Holder of the Mouth of Madness and Mismemory which allows him to reshape people's memories as a personal power. 
Jack Truman (Agent 18/Deathlok) – Fourth known  Deathlok cyborg.
Samantha Twotrees – Turncoat working with rogue agent Inali Redpath. 
Steven Tyler – Infiltrated the City of the Space Gods. He was reduced to basic matter. Rank L-6 
Wendell Vaughn (Quasar) – Became the superhero Quasar during a security assignment. 
Kali Vries – Former lover of  John Walker. She infiltrated S.T.A.R.S. (Superhuman Tactical Activities Response Squad).
John Warden – Lead ESP telepath stationed at telepathic amplifier. 
Seth Waters – Administrator for Washington, D.C.'s bureau. 
Commander Jonas Williams – Led task-force to apprehend  AWOL agent, Sharon Carter.
Sam Wilson (Falcon) – Superhero and  Avengers member the Falcon.
Annie Wong - Agent from S.H.I.E.L.D.'s Hong Kong branch. 
Jimmy Woo (Yellow Claw) – Former  FBI agent who fought Yellow Claw and later hunted Godzilla. Level 3 interrogator and Level 5 agent (two transcripts of Nick Fury's "Secret Files" in list Jimmy Woo as a Level 8 agent). Secret War Larry Young – S.H.I.E.L.D.  Air Cavalry officer. Later, fifth known Deathlok cyborg.
Notable Agents (Post-Civil War)
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adding to it
. (March 2014)
Civil War, all super-heroes that fought on the Pro-registration side of the conflict are now agents of S.H.I.E.L.D.
Val Adair – Rank L-6; expelled. 
Scott Adsit – A former L-6 agent who left with other agents to aid in the reformation of the Nova Corps. The character is based on real life comedic actor Scott Adsit.
Agent 9 – Rogue agent who took over the Helicarrier for the  Red Skull. Agent 9 was later killed.
Paul Allen – Rank L-6 agent. He was expelled from S.H.I.E.L.D. 
Harry Angstrum – Records-division agent. He was devoured by Biohazard. 
Dr. Belgrade – Chief scientist in charge of Nemesis project. He was killed by his own creations. 
Yelena Belova (White Widow) – Second known Black Widow. 
Cameron Bissett – Ensign who was killed by  Sabretooth. He was later impersonated by Mystique.
John Bronson – Killed and identity taken by  Wolfgang von Strucker. Rank L-4
Laura Brown – Daughter of  Imperial Hydra (Arnold Brown) and one-time lover of Nick Fury.
Peggy Carter – Rank L-6; deceased. 
Stanley Carter (Sin-Eater) – Became the Sin-Eater and killed  Jean DeWolff.
Colonel Kuro Chin (Agent 60) – Colonel in the Yashonka military. Shot dead helping  Captain America escape from that Communist country.
Clayton Claymore – Cyborg. Died on mission to El Corazon de la Muerte. 
Edward Cobert (Gargantua) – Became the Leviathan when his project went wrong. 
Jeff Cochren – Rank L-5; deceased. 
Condor – On black-ops team the Contingency. Turned rogue and was captured. 
Corbin – Surveillance. He was later assigned inventory duty at Virginia storage center. Killed by an undercover A.I.M. agent.  
Edwin Darwin – Cyborg. Died on a mission to El Corazon de la Muerte 
Alex DePaul – Led investigation of Deviant warlord  Tantalus. Friend of Bison. Killed by agent Kara Palamas. 
Carl Delandan (sometimes misspelled Karl) – Regional Director in Manhattan. Rank L-3. He was dismissed from S.H.I.E.L.D. 
Phil Dexter – Retired. 
Roger Dooley – Level 4 officer who usurped Dum Dum Dugan's rank and used the Helicarrier to capture  She-Hulk. He was killed.
David Ferrari (Answer) – Toxic-weapons division at the Furnace. He was thought dead, but was later revealed alive and known as Answer. 
Marvin Flumm (Mentallo) – Psi-division. After being discharged, he became the  mutant terrorist Mentallo.
John Garrett  – Cybernetically enhanced agent, known for his lack of discipline. Later becomes the President of the United States of America by the unwanted help of  Elektra, who put his mind into the body of the (fictional) President Ken Wind, in order to save the world.
Lt. Tom Gittes – Auxiliary CO and security chief on the Helicarrier. He was among the group of renegade agents attempting to take over Helicarrier for impromptu  war crimes trial of KGB agent. He was killed by agent Thurman.
Dr. Erik Gorbo – Scientist. He changes form into that of a gorilla to commit crimes. 
Hardcase and the Harriers – Mercenaries who are former agents of S.H.I.E.L.D. 
Margaret Huff – Nick Fury's personal secretary. She reassigned to S.H.I.E.L.D.'s Los Angeles branch where she died investigating a cult. Rank L-6 
Brent Jackson – Betrayed S.H.I.E.L.D. and was a mole for  Weapon X.
Major Shera Joseph – Los Angeles branch member who headed the task force in Imaya.  Joined Worldwatch and died of time-travel fatigue.  
Sal Kennedy - Old friend of Tony Stark's, took the position when he became director, killed in an attack on the Helicarrier 
Killdeer – On black-ops team the Contingency. Turned rogue and was captured. 
Kite – On black-ops team the Contingency. Turned rogue and was captured. 
Kevin Kraller – Rank L-6; deceased. 
Jeremy Latcham – Double agent for the  Purifiers. He was self-terminated.
Scott Niles Lawrence – Rank L-6; deceased. 
Shannon Lawrence – Undercover agent exposed to mutating virus giving her ability to shoot knives from her fingers. She was killed.  
Elizabeth Lockhart – High-ranking budget officer.  Leaked information to the  Punisher; liquidated by the Contessa. 
James "Jamie" Madrox (Multiple Man) – One of several duplicates of the  mutant Jamie Madrox (Multiple Man). He was re-absorbed by the original Madrox.
Adam Manna – Rank L-6; expelled. 
Barbara Morse (Mockingbird) – Known as Agent 19. Rank L-6 agent. She resigned from S.H.I.E.L.D. She was later killed by  Mephisto. Barbara was revealed to be alive and replaced by a Skrull.  Level 6 agent. 
Dani Moonstar (Mirage) – Former  New Mutant. Undercover agent infiltrating the Mutant Liberation Front. She resigned following Operation: Zero Tolerance.
David Nanjiwarra – Aboriginal agent of  A.S.I.O. He was used as a mole and subsequently killed by Scorpio.
"Network" Nina – Cyber-enhanced ESP agent that fought Algernon Crowe. She was killed in telepathic battle with Psi-Borg.  
Kate Neville – Chief of Ordnance and former lover of Nick Fury, after he was ousted by a compromised S.H.I.E.L.D. by Prtoject Delta-  LMDs. She was killed by Baron Strucker. 
Arthur Perry – Unscrupulous killer who made it into a S.H.I.E.L.D.- program for cybernetically enhanced "super agents". Was later killed by  Elektra.
Kitty Pryde – Member of the  X-Men as Shadowcat. Recruited as an intern while a member of Excalibur.
Dave Purcell – Killed with girlfriend and identity taken by HYDRA at academy graduation ceremony. 
Pyle – Surveillance-team leader. He was later assigned inventory duty at Virginia storage center where he was killed.  
Rapture – Leader of the black-ops team the Contingency. Rapture's a low-level telepath. 
Inali Redpath –  Cherokee shaman with ability to control weather. He turned rogue and used S.H.I.E.L.D. to attack U.S. to reclaim land for the Native Americans.
Richard Rennselaer – Field agent (L-6); resigned. He became the  mutant terrorist Overrider.
Frank Rhodes – Quit S.H.I.E.L.D. and eventually became an ally of  Cable. Killed by D'Von Kray, a New Canaanite warrior.
Buck Richlen – Rank L-5; expelled. 
Nathaniel Richards 
Rico Santana – Rank L-3; expelled. 
Heather Sante – Charged by Iron Man to keep an eye on Mar-Vell. 
Simon – Double agent for  Tantalus. He was killed by Pandara. 
Shrike – Member of the black-ops team the Contingency. He turned rogue and was captured. 
John "Skul" Skulinowski – Team leader of the Elite Agents. 
Eugene Spandell – Cyborg. He died on mission to El Corazon de la Muerte. 
Howard Stark 
SULTAN – Former weapons designer and computer and code expert. 
– As Warhawk, he was conscripted to serve as a temporary agent in exchange for reducing his sentence. Mitchell Tanner
Neal Tapper – Formerly involved with Sharon Carter. He was killed in an explosion. 
Kate Waynesboro – Monitored Bruce Banner following Banner's presidential pardon.  William Wesley – Test pilot who volunteered to test the  quantum bands, which overloaded and killed him.
Blue Streak – Member of the first Super Agents program. He was a double agent for the corporation. Assassinated by  Scourge of the Underworld.
Wendell Vaughn (Marvel Boy) – Member of the first Super Agents program. He later became known as "Quasar". 
Texas Twister – Member of the first Super Agents program. Quit S.H.I.E.L.D. to form his own team, the  Rangers.
Vamp – Member of the first Super Agents program. Professional criminal who is a double agent for the corporation. Became "Animus". Killed by  Scourge.
Ivory – Wakandan native in the second Super Agents program.  She was killed by HYDRA double agents.  
Knock-About – HYDRA double agent. Imprisoned by Nick Fury.  
Psi-Borg – Cybernetically enhanced  Latverian. She is a charter member of the second Super Agents program. Revealed as a HYDRA double agent and was killed in  telepathic battle with "Network" Nina.  Violence – She was revealed as a HYDRA double agent in the Super Agents program. Granddaughter of ex-Howling Commando Percy Pinkerton. Killed by Nick Fury.  
As revealed in the 2010 series
Marvel Cinematic Universe S.H.I.E.L.D.
In the Marvel Cinematic Universe, S.H.I.E.L.D. was founded by Strategic Scientific Reserve Agent Peggy Carter, along with Howard Stark in 1965. Howard Stark is the deceased father to
Tony Stark which is why Iron Man has supported the group, until it was revealed in , that S.H.I.E.L.D. was infiltrated by Captain America: The Winter Soldier Hydra since its formation. When Hydra emerges in 2014, S.H.I.E.L.D. is labeled as a terrorist organization while most of its non-Hydra members leave S.H.I.E.L.D. to work the private sector or go off the grid. In the second season of the television series , after Nick Fury charges him with rebuilding the organization, Phil Coulson manages to win back the American government's trust as S.H.I.E.L.D. now works behind the scenes. S.H.I.E.L.D. is currently defunct after the destruction of The Playground, S.H.I.E.L.D.'s HQ and the mysterious disappearance of Former Director Philip J Coulson.
Agents of S.H.I.E.L.D.
Executive directors and secretaries
– co-founder of S.H.I.E.L.D.; First known executive director. Deceased . Peggy Carter
- co-founder of S.H.I.E.L.D.. Deceased. Howard Stark
- co-founder of S.H.I.E.L.D.. Deceased. Chester Phillips
- Secretary of S.H.I.E.L.D.. Deceased. Alexander Pierce
- Former executive director; faked his death after S.H.I.E.L.D's fall and appointed Phil Coulson as his successor. Nick Fury
- Field agent; former deputy director; had Level 9 Security Clearance before S.H.I.E.L.D. was taken down. Maria Hill
- Executive director; current field officer who oversees many of the division's field operations; briefly deceased before being subjected to GH-325. Organic body has deceased but his consciousness was transferred to an LMD based on Phil Coulson Chronicom technology.  
- Executive director. Appointed in the wake of the Jeffrey Mace Sokovia Accords and S.H.I.E.L.D. being made a legitimate organization again. Deceased.
- Field agent; computer hacker; first known Inhuman agent with the ability to generate seismic vibrations. Leader of the Secret Warriors. Former executive director of S.H.I.E.L.D. Daisy Johnson (Skye, Quake)
- Mechanic and field agent; founding member of the "Real S.H.I.E.L.D." and new executive director of S.H.I.E.L.D. handpicked by Daisy after Coulson retired. Alphonso "Mack" MacKenzie , The new executive director of S.H.I.E.L.D. in the alternate reality Deke Shaw
Agent 60 - In contact with Coulson's team.
Barbour — Stationed at the Hub.
Baylin — Stationed at the Hub.
Tomas Calderon — Member of the "Real S.H.I.E.L.D."
Goodman — Doctor; participated in experiments on GH-325 and in Phil Coulson's resurrection.
Billy Koenig — Stationed at secret base Playground.
Sam Koenig — Stationed at secret base Playground.
Jazuat — Doctor; stationed at S.H.I.E.L.D. Trauma Zentrum in Zurich, Switzerland.
Jones — Stationed at the Hub.
Mack — Undercover trucker.
Mike Peterson ( - Former test subject for Project Centipede and slave of John Garrett and Hydra. Now a member of S.H.I.E.L.D. Deathlok)
- Inhuman field agent with ability to move at super speed for the duration of one heartbeat before returning to her starting point. Member of the Secret Warriors. Elena "Yo-Yo" Rodriguez
Katherine Shane — Undercover specialist.
Shade — Stationed at the Hub.
Shaw — Undercover specialist; stationed at the Hub.
Streiten — Doctor; participated in Phil Coulson's resurrection.
Tyler — Mechanic aboard helicarrier; later field agent. Anne Weaver — Director of the S.H.I.E.L.D. Academy of Science and Technology; member of the "Real S.H.I.E.L.D."
Akela Amador — Undercover operative prior to being kidnapped by Project Centipede/Hydra organization. Freed from their control by Phil Coulson's group. Incarcerated.
Linda Avery — Field agent who discovered Skye as an infant. Deceased.
- Assassin and skilled marksman with an exceptional ability in archery; founding member of the Avengers. Level 7 Security Clearance. Clint Barton (Hawkeye)
- Espionage agent. Laura Barton (Agent 19)
Felix Blake — Espionage agent; went rogue after S.H.I.E.L.D.'s collapse and joined the Watchdogs. Status Unknown.
Roger Browning — Stationed at a S.H.I.E.L.D. containment facility; went freelance after S.H.I.E.L.D.'s fall; killed by Carl Creel while trying to sell classified information.
- Inhuman doctor and field agent with ability to generate electrical charges; member of the Secret Warriors. Deceased. Lincoln Campbell
- Former Head of Defense at S.H.I.E.L.D.; now a member of Hydra. Mitchell Carson
- Espionage agent; now a member of the CIA. Sharon Carter (Agent 13)
Chaimson — Stationed at the Hub. Deceased.
Sebastian Derik — Assassin; test subject for GH-325; arrested after killing other GH-325 test subjects.
- Weapons, gadgets, and cutting-edge technology, Level 5 Clearance; Leopold "Leo" Fitz Engineer  
- Scientist; fired by Hank Pym. Bill Foster
- Field operative; Hydra double agent known as the "Clairvoyant"; prototype John Garrett Deathlok. Deceased.
Robert Gonzales — An elderly high-ranking S.H.I.E.L.D. Agent, tactician, commander of the Iliad, and founder of the "Real S.H.I.E.L.D."; Deceased.
- Former S.H.I.E.L.D. instructor; discovered gravitonium. Franklin Hall
Joey Gutierrez — Inhuman construction worker and field agent with ability to manipulate metal objects; member of the Secret Warriors.
- Director of the Hub. Deceased. Victoria Hand
Isabelle "Izzy" Hartley - Undercover specialist; member of "Real" S.H.I.E.L.D. Deceased. 
Hauer — Hydra double agent in 1989; freed Werner Reinhardt from custody.
- Decorated former member of Lance Hunter SAS who joined his old friend Agent Hartley in doing mercenary work for Coulson's reconstituted S.H.I.E.L.D.; formally joined S.H.I.E.L.D. after avenging Hartley's death. Disavowed status.
Kaminsky — Helicopter pilot; Hydra double agent. Presumed deceased after being buried in a collapsing Hydra base by May.
- Field agent; test subject for GH-325; had memories erased and retired to civilian life. Cameron Klein/Hank Thompson
- Communications agent; stationed at hidden base Providence. Deceased. Eric Koenig
Jacobson — Stationed at the Hub. Deceased.
Richard Lumley — Field agent who discovered Skye as an infant; faked death and went off the grid.
- Ace pilot and weapons expert, Level 7 Security Clearance, a.k.a. "the Cavalry".; Retired. Melinda May Administrator  
Barbara "Bobbi" Morse - Espionage agent; founding member of the "Real S.H.I.E.L.D."; a.k.a. Mockingbird. Disavowed status. 
Oliver — Member of the "Real S.H.I.E.L.D." Deceased.
Kara Lynn Palamas (Agent 33) - Espionage agent; brainwashed by Daniel Whitehall. Deceased.
- Bank robber recruited into S.H.I.E.L.D. R&D Think Tank. Whereabouts unknown. Benjamin "Benny" Pollack
- Scientist and field operative. Hank Pym (Ant-Man) 
Quan Chen — Field agent of S.H.I.E.L.D.. Deceased.
Jack Rollins — Member of S.T.R.I.K.E.; Hydra double agent. Whereabouts unknown; presumed deceased.
- Assassin and undercover spy; founding member of the Avengers; one of the few agents with known Level 10 Security Clearance. Deceased. Natasha Romanoff (Black Widow)
- Field operative; leader and founding member of the Avengers. Level 8 Security Clearance. Whereabouts unknown; likely deceased. Steve Rogers (Captain America)
- Leader of Brock Rumlow S.T.R.I.K.E.; Hydra double agent. Deceased.
Lewis Seaver — Test subject for GH-325. Deceased.
- Astrophysicist; originally recruited for Project P.E.G.A.S.U.S. to study the Tesseract. Erik Selvig
- Life scientist; Xenobiologist (both human and alien), Jemma Simmons Level 5 Clearance, Bio-chemist.  
- Espionage agent; Hydra double agent. Deceased. Jasper Sitwell
- Chief of S.H.I.E.L.D. West Coast in the 1950s. Originally deceased but was saved by Phil Coulson. Joined Daisy Johnson. Daniel Sousa
- S.H.I.E.L.D. Advisor; founding member of the Avengers. Deceased. Tony Stark (Iron Man)
- Former field agent. Whereabouts unknown. Ava Starr (Ghost)
- Scientist; fired by Hank Pym. Deceased. Elihas Starr
- Stationed at the Triskelion; test subject for GH-325. Deceased. Rebecca Stevens/Janice Robbins
Antoine "Trip" Triplett — Field operative. Deceased.
- Scientist and field operative. Janet Van Dyne (Wasp) 
- Black ops specialist; Level 7 Security Clearance; Hydra double agent. Deceased. Grant Ward 
Noelle Walters — Team leader in Europe. Deceased.
- Accomplice of Benny Pollack; became apprentice of Agent Felix Blake. Whereabouts unknown. Claire Wise - Hydra scientist recruited during Arnim Zola Operation Paperclip following World War II; restarted Hydra within S.H.I.E.L.D. Deceased.