The 1996 limited series Kingdom Come by Mark Waid and Alex Ross presents a Batwoman portrayed as a Batman admirer from Jack Kirby's Fourth World. Her costume mixed Kirby-esque elements with those of the original costume. The character rode a giant bat-winged dog named Ace.
Batman: Dark Knight Dynasty (1997) features Vice-President Brenna Wayne who becomes Batwoman in order to stop Vandal Savage. Wayne discovers a conspiracy against her family after investigating the last thirteen generations of the Wayne family.
Superman/Batman #24 (November 2005) presents a world where the genders of the characters are reversed with Helena Wayne as Batwoman. DC later placed this alternate reality as "Earth 11" in the post-Infinite Crisis multiverse.
Flamebird assumes the mantle of Batwoman in "Titans Tomorrow". Art by Mike McKone.
Kate Kane as Batwoman debuted in 2006 in the series 52.
In the Teen Titans storyline "Titans Tomorrow" (2005), Bette Kane is Batwoman, and wears a costume similar to Kathy's pre-Crisis one. In the follow up storyline, "Titans of Tomorrow... Today!" (2007), Bette Kane remains Flamebird and former Batgirl Cassandra Cain becomes Batwoman.
Katherine Webb, a member of the Kane family and the aunt to both Kate and Bette, is the Headmistress of Pinkney Orphanage. She was left in charge of the orphanage by Kate before she left Gotham. Headmistress Webb is a xenophobic woman, who uses the orphans to make robots in order to protect the country from foreigners, whom she characterizes as evil. She is later defeated by a team of Batgirls, including Bette, who come to the orphanage to save the orphans.
Kathleen "Kathy" Duquesne, an auto mechanic and the leader of a team of Batgirls, who protect the city during Batwoman's absence. Along with Kathy, the team includes Nell Little, Harper Row and later on Alysia Yeoh and Bette Kane. Upon meeting Bette, Kathy immediately recognizes her as a baseball player and later figures out her real identity as well as her connection to Batwoman but promises to keep it a secret. The Batgirls save the orphans of Pinkney Orphanage from the devious Headmistress and later expand their team, welcoming Tim Drake, Cullen Row and Felicity Smoak, and creating a Bat family.
Sonia Alcana appears as an officer of Gotham City Police Department, partnered with Crispus Allen and working with Maggie Sawyer to aid Batwoman into her fight against crime in the city.
A loose adaptation of Batwoman appears in the Batman: The Brave and the Bold episode "The Criss Cross Conspiracy!", voiced by Vanessa Marshall. This version is visually based on the Kathy Kane incarnation, but is depicted as series original character Katrina Moldoff / The Bat Lady, thrill-seeking heiress to the Moldoff Circus fortune and a trained circus acrobat whose crime-fighting style endangers bystanders. After being publicly unmasked and humiliated by the Riddler years prior, she is forced to give up her crime-fighting lifestyle before seeking revenge in the present by encouraging Felix Faust to give her a spell to switch her and Batman's bodies, allowing her to get closer to the Riddler without the police coming after her. After she fails, Batman rescues her and convinces Faust to switch them back before she quietly turns herself into the police. Series director Ben Jones confirmed that the decision to rename the character was brought about after DC Comics voiced concerns about this depiction of the character having a negative impact on the new Batwoman comic book series, the first issue of which launched less than a month after the episode's initial air date.
A variation of Batwoman appears in the DC Animated Universe film Batman: Mystery of the Batwoman, voiced by Kyra Sedgwick. This version is Gotham Police Detective Sonia Alcana (voiced by Elisa Gabrielli), Wayne Tech employee Dr. Roxanne "Rocky" Ballantine (voiced by Kelly Ripa), and Kathleen "Kathy" Duquesne (voiced by Kimberly Brooks), who all hold grudges against the Penguin and Rupert Thorne, and Carlton Duquesne, a gangster in the crime bosses' employ and Kathy's father. Alcana, Ballantine, and Kathy pooled their resources and skills together and take turns operating as Batwoman to draw suspicion away from each other, with all three using lethal force to achieve their goals. In the DVD and Blu-ray special features, the producers explain that DC did not want the filmmakers to use Kate Kane in a family-friendly movie due to the violence associated with her character.